Composite

Part:BBa_M36158:Design

Designed by: Sohaib Shaikh and Alfonso Ocampo   Group: Stanford BIOE44 - S11   (2014-10-23)


E.coli Temperature Shock Sensor/Actuator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 656
    Illegal AgeI site found at 764
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since we wanted to design E.coli that would produce color under cold shock and heat shock, the this part was designed such that after the heat shock promoter, the gene for Comet would be placed. This is only achievable if the DNA backbone of E.coli contains Comet.


Source

See subparts below for part sources.

References